Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Mechanical properties of extracellular environment are important in differentiation of neuronal stem cells and maturation
of neurons. However, which genes are involved in mechanobiology of neurite outgrowth remains largely unknown.
Family with sequence similarity 134 member C (Fam134c) and chromosome 3 open reading frame 10 (C3orf10) are two
novel genes differentially regulate axonal and dendritic growth in rat hippocampal neurons. Fam134c is predicted as a
membrane protein and C3orf10 is proposed to involve actin nucleation. Focal adhesion kinase (FAK) is a key molecule in
mechanosensitivity. We hypothesized that Fam134cand C3orf10 involved in mechanobiology of neurite outgrowth under
different rigidity of matrix and are up or downstream signaling molecules of FAK. Results showed that primaryrat hippocampal
neurons exhibit the rigidity-dependent increases in the length of axons. Knockdown of Fam134c decreases the length of axons
on glass dish but increases the length of axons on soft gel. In contrast, knockdown of C3orf10 increases the length of primary
dendrites on rigid matrix. Immunointensity of both Fam134c and C3orf10 in cell bodies showed rigidity-dependent decrease.
However, immunointensity of Fam134c in axons increases on soft gel but that of C3orf10 has no difference on different rigidity.
Immunointensity of phosphorylated FAK (pFAK) exhibits rigidity-dependent increases in primary rat hippocampal neurons.
Fam134c and C3orf10 colocalize with pFAK and paxillin on different rigidity of matrix. Knockdown of C3orf10 decreases the
expression of pFAK on rigid matrix but not on soft matrix. These results provide findings that novel gene Fam134c and C3orf10
play differential roles in mechanosensing of neurons probably through the interaction with FAK.
Biography
A-Min Huang has completed his PhD from National Defense Medical Center, Taipei and Postdoctoral studies from Institute of Biomedical Sciences, Academia
Sinica, Taipei, Taiwan. She is now an Associate Professor in the Department of Physiology, College of Medicine; National Cheng Kung University, Taiwan. She has
published more than 20 papers in reputed journals in the neuroscience field.
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals