Toxicology: Open Access
Open Access

Abstract

Currently, various wound healing agents are being developed. The patch-type therapeutic agent for skin wound healing has almost no side effects and the drug is delivered to the body at a constant rate, it is a new concept drug treatment that can be applied directly. Most of the conventional patch type therapeutic agents have limitations in that the drug is not well controlled due to the structure of the film, so that the drug is not smoothly absorbed into the skin and the secondary infection is a concern due to poor ventilation and sterilization. In this work, nanofiber sheets (NS) as patch-type therapeutic agent using small intestinal sub-mucosa (SIS) prepared by electro-spinning. Nano-fibers have a large surface area and high porosity. The large surface area not only sufficiently constitutes the wet state but also has a three-dimensional structure similar to the extracellular matrix structure (ECM), so that drug release is facilitated at the wound site and effective for skin regeneration. Also, SIS, a natural material, has excellent biocompatibility and is an ECM with signaling bio-active molecules, cytokines and many other factors, so it has the advantage of controlling cell function. In this experiment, the nanofiber morphology of the SIS-NS was evaluated by SEM. The absorbability of SIS-NS was evaluated by contact angle test. The cytotoxicity of SIS-NS was evaluated and the wound healing effect was confirmed in vitro. Also, the skin wound healing effects of SIS-NS was evaluated by in vivo experiments such as incision and staining for 3 weeks. In conclusion, these results indicate that SIS-NS is effective for skin wound healing and SIS-NS can be applied as a therapeutic agent for various types of skin wound healing effects.

Biography

Minju Kim is currently studying at Regenerative Medicine Laboratory in Ajou University. Her main research interests are drug delivery system.
Email:goroki42@ajou.ac.kr