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Monoclonal antibodies against envelope glycoprotein of yellow fever virus
World Congress on Infectious Diseases
Woo Young Choi, Seok-Min Yun, Eunbyeol Wang, Young Eui Jeong, Myungguk Han and Won-Ja Lee
Posters-Accepted Abstracts: J Infect Dis Ther
Abstract
Yellow fever virus (YFV) belongs to family Flaviviridae and is important mosquito-borne human pathogens. In this study,
we describe the generation of monoclonal antibodies (mAbs) against envelope glycoprotein (E) of 17D Yellow fever
vaccine virus for serologic diagnosis. After immunization of BALB/c mice with recombinant E protein, a total of 5 monoclonal
antibodies were generated from the spleen of mice. Immunoglobulin types of antibodies were found to be 1 IgG2a/kappa,
2 IgG2b/kappa, and 2 IgG1/kappa. Indirect immunofluorescence assay revealed that all of the mAbs were reactive to YFVinfected
cells. These data suggest that 5 monoclonal antibodies can be used for detection of YFV and have the potential for use
in serodiagnosis.
