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In Association with
Molecular screening of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium and Ureaplasma urealyticum infection with infertility patients in north India
6th World Congress on Biotechnology
Amit Roy, Rakesh Yadav, Rajneesh Dadwal, Anuradha Chakraborti, L K Dhaliwal, Shivaprakash M Rudramurthy, Shalini Gainder and Sunil Sethi
Post Graduate Institute of Medical Education and Research, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Infertility in female is one of the most important sequelae of sexually transmitted infections (STIs). Neisseria gonorrhoeae and Chlamydia trachomatis are the predominant agents causing infertility. Pathogenesis of Mycoplasma genitalium and Ureaplasma urealyticum remain to be addressed. Asymptomatic carrier is serious epidemiological problem because of unconscious infection to sexual or perinatal transmission. Therefore accurate and timely diagnosis is essential for treatment to prevent transmission and infertility. Advantage of real-time PCR (RTPCR) is to combine detection and quantitation simultaneously. The aim of study was to screen N. gonorrhoeae, C. trachomatis M. genitalium and U. urealyticum in infertile and healthy women. 248 women (98 infertile and 150 healthy) attending the gynaecology clinics were included in the study after excluding other causes of infertility. ECS and endometrium tissue were tested by RTPCR using gene PorA of N. gonorrhoeae, cryptic plasmid of C. trachomatis, MgPa of M. genitalium and urease of U. urealyticum. Sequencing was done on these specimens that were positive by RTPCR to confirm the results. Of 98 infertile patients, the prevalence of N. gonorrhoeae, C. trachomatis M. genitalium and U. urealyticum were 8.1%, 10.2%, 7.1% and 46.9% respectively. Significantly, PID and BOH were associated with infertility. Co-infections have been found in 1% to 5% among the organisms. Of 150 healthy women prevalence of M. genitalium and U. urealyticum were 5.3% and 14% respectively. ECS was found to be sensitive specimen. Therefore RTPCR in ECS could be helpful for diagnosis and screening in reproductive age group. These findings may be useful to guide therapy, prevent transmission and infertility.Biography
Email: amitroy.micro@gmail.com
