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Molecular characterization of Pythium spp. isolated from tomato seedlings in the Syrian coast
8th Euro Biotechnology Congress
Mohammad Imad Khrieba1,2, Wafaa Choumane2, Ibtissam Ghazal2 and Fawaz Azmeh1,3
1National Center for Biotechnology, Syria 2Tishreen University, Syria 3Damascus University, Syria
Posters-Accepted Abstracts: Biotechnol Biomater
Abstract
Tomato seedlings damping-off is a limiting factor in commercial greenhouse production. To determine the causal agents of disease, sampling and fungal isolation were performed during 2012. Samples were collected from infected seedlings growing in greenhouses in the Syrian coastal region, isolation of fungi was done in the laboratories of the Agronomical Research Center in Lattakia and the molecular analyses were done in the Biotechnology Center at Tishreen University, Lattakia, Syria, during the year 2012-2013. Eight isolates of Pythium sp. obtained were purified using hyphal tip method (named P1, P2, P3, P4, P5, P6, P7 and P8). Isolates were morphologically identified by optical microscope then molecularly characterized using genus specific ITS primers. The results of morphological characterization of pathogenic species suggested the detection of Pythium aphanidermatum, P.ultimum. The analysis of DNAs from the different isolates with ITS primers recognizing the inter transcript spacer of nuclear ribosomal DNA proved that the eight isolates were belonging to the species P. ultimum. The complete sequences of ribosomal DNA internal transcribed spacers regions of selected isolates were determined and submitted to GenBank. The GenBank-BLAST homology search revealed P. ultimum as the most similar sequence (>99% identity) with GenBank entry AB355596.Biography
Mohammad Imad Khrieba got a Doctorate in 2015 from Tishreen University in Syria, Faculty of Agriculture, in the field of biological control of plant diseases and interpretation of the effect by using the methods of biotechnology mechanism. He got a Master’s degree from Isfahan University of Technology in Iran and almost within the same area of his Doctorate in 2011. He has participated in many scientific conferences inside Syria and abroad and published during the Master’s and Doctoral studies, several articles in scientific journals. He is currently working as a Researcher at the Public Authority for Biotechnology in Damascus.
Email: imadkhrieba@gmail.com
