Journal of Biotechnology & Biomaterials
Open Access

Abstract

Bakanae is caused by Fusarium fujikuroi (Nirenberg) is emerging as a serious disease of rice in India. Elongation and rotting of seedlings are distinguished symptoms of bakanae disease. Sixty three isolates of Fusarium fujikuroi were isolated from symptomatic diseased plants collected from Jammu and Kashmir, Punjab, Haryana, Uttar Pradesh, Uttarakhand and Bihar. Identities of the isolates were confirmed through translation elongation factor 1-�± (tef-1�±) gene sequence based identification. Out of 12 Universal Rice Primers (URP) used in the study, six primers effectively produced polymorphic bands in all the isolates. URP 17R and URP 6R primer produced 93% polymorphic bands. Mating type of the population was identified based on MAT-1 and MAT-2 region universal primers (GFmat1a, GFmat1b, GFmat2c and GFmat2d) for Gibberella fujikuroi. Among the 63 isolates 18 (28.57%) were identified as MAT-1 and 45 (71.42%) as MAT-2. Effective population number Ne(mt) for mating type was 89% of the count (total population). Since both the mating types were not present in equal frequencies in the F. fujikuroi population, asexual reproduction was likely to occur in the field isolates. However, the occurrence of both the mating types in the F. fujikuroi implied that the population members are capable of sexual reproduction. Therefore, it is important that programs to develop cultivars with the bakanae disease resistance considering other factors including the environmental conditions and variability of the pathogen in the area of intended cultivation.

Biography

Bishnu Maya Bashyal has completed her PhD from Banaras Hindu University. She is working as a Scientist at Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi. She has published more than 25 papers in reputed journals.

Email: bishnumayabashyal@gmail.com