Journal of Biotechnology & Biomaterials
Open Access

Abstract

Molecular and genetic studying of lactic acid bacteriaâ??s (LAB) gene pool in depositaries is an important problem for the purpose of their wide use in the food industry. Thus control of genome stability in the course of storage is necessary. In research used 17 strains of the genes Lactobacillus and Bifidobacterium allocated from a grain, flour, milk and the dairy production, being stored in a gene bank in the lyophilic-dried-up look within 10 years. Specific identification of strains is carried out on the basis of PCR of 16 S RNA gene. The purpose of researches â?? to study molecular and genetic stability of bacterial cultures at long storage with use of 9 ISSR markers: M2-(ac)8ctg, M3-(ga)8(ct)c, M4-(ag)8(ct)c, M5-(at)8, M7-(cag)5, M8-(gtg)5, UBC 840-(ga)8ayt, UBC 855â??(ac)8cyt, UBC 881 â?? ggg-tgg-ggt-ggg-gtg. Allele polymorphism of ISSR loci is studied, all is received 396 amplicones. It is established that after 10 years of storage at 4 strains of LAB were observed changes in a genome in size fragments of DNA from 700 to 2000 c.n. Biochemical researches showed that changes in DNA of LAB didn't mention the structural genes influencing, for example, on the one of phenotypic trait â?? activity of acidformation. All studied strains during ten years' storage kept the activity and a high titer of cages at one level.

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