Journal of Biotechnology & Biomaterials
Open Access

Abstract

Rice (Oryza sativa L), is the most important food crop for over half of the world�s population and supplies 20 percent of daily calorie intake. Salinity is one of the major abiotic stresses that adversely affect crop productivity and quality. Rice is found to be sensitive to soil salinity with salinity threshold level of 3.0 dS m-1 and 12 percent reduction in grain yield per degree increase in electrical conductivity (EC) beyond this threshold value. The problem of soil salinity is further increasing because of the use of poor quality water for irrigation and poor drainage. The yield reduction in salt soils can be overcome by soil reclamation or by improving salt tolerance in target crops. The use of molecular markers, permit the genetic dissection of the progeny at each generation and increase the speed of the selection process. Molecular markers could be used to tag quantitative trait loci (QTLs) and to evaluate their contributions to the phenotype by selecting favorable alleles at these loci in a marker-assisted selection scheme. Of the available molecular markers, microsatellite markers (SSRs) being co-dominant, highly polymorphic and economically assayed by PCR have been preferentially used for genotyping and genetic diversity analysis in rice. Seeds harvested from Pusa1121��CSR10 F1 plants are being used in the present study. The seeds are being treated initially at 25-50 mM of NaCl and plants showing resistance towards salt stress are being carried forward to next generation. The selected plants would be also tested for BAD2 locus specific for aroma as well as an array of SSR�s would be used to help in the detection of QTL�s related to salt tolerance.

Biography

Email: mahaveer.bishnoi9@gmail.com