Journal of Biotechnology & Biomaterials
Open Access

Abstract

Tribulus terrestris has an ancient tradition in folk and in Ayurveda as a diuretic, antiseptic and anti-inflammatory agent, it is also a common constituent of various marketed antiurolithiatic herbal formulations like Uriflow, Uritone, Cystone, Neeri and Culdisol, etc. To investigate the effect of aqueous extract (AE) of Tribulus terrestris of different extraction parameters i.e., AE1 (temp. 23.50�°C, time 19.50 hours and S:L 12:00), AE2 (temp. 4.16�°C, time 19.50 hours and S:L 12:00), AE3 (temp. 35�°C, time 36 hours and S:L 12:00) and AE4 (temp. 35�°C, time 3 hours and S:L 20:00) on calcium oxalate crystals (COM) formation, crystals size and morphology of crystals, polarization microscopic imaging technique was used. To investigate the effect of test sample, 50 �¼l of CaCl2 (4.25 mM), 50 �¼l of Na2C2O4 (0.75 mM) and 50 �¼l of test samples were mixed and incubated at 37�°C for 45 minutes. Crystal morphology was examined in five randomly selected fields at 40�� magnification from different fields under an upright microscope (Olympus Corporation, Japan). Microscopic analysis showed that the aqueous extract AE1 and AE2 reduces the number of crystals, shine of crystals and also showed distortion in morphology of COM crystals as compared to AE3 and AE4. In conclusion, AE1 and AE2 found more effective then AE3 and AE4.

Biography

Jyoti Kaushik has completed her MSc Biotechnology from Lovely Professional University, Punjab. She is currently pursuing her PhD in Biotechnology and working as SRF in DBT sponsored project under the guidance of Dr. Chanderdeep Tandon from Amity Institute of Biotechnology, Amity University, India.

Email: jkaushik39@gmail.com