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In Association with
Microsatellite markers studies for genetic characterization of Bikaneri camel
6th World Congress on Biotechnology
Lokesh Gautam, Hina Ashraf Waiz and Kavita Tyagi
Rajasthan University of Veterinary and Animal Sciences, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Genetic variation in Bikaneri camel was analyzed using eleven microsatellite primers. A total of eleven microsatellite primers were tested for amplification on a panel of 30 unrelated camels. Polymerase chain reaction (PCR) was employed for amplification of eleven microsatellite loci. The PCR amplified products were separated by denaturing gel electrophoresis. The gel was stained with silver nitrate procedure. The allele frequency, expected heterozygosity and polymorphic information content were observed. Six out of eleven (54%) microsatellite primer pairs gave a polymorphic band. The number of alleles ranged from two to five in Bikaneri camel for different microsatellite markers. The expected heterozygosity ranged from 0.289 (VOLP -08) to (VOLP-10) and the polymorphic information content values in camel ranged from 0.267 (VOLP) to 0.639 (VOLP-10). On the basis of all over observation, it was revealed that microsatellite primers were found to be polymorphic with good polymorphic information content. Hence they can be used for further genetic studies.Biography
Lokesh Gautam is currently working as an Assistant Professor as well as pursuing his PhD in the Department of Animal Genetics & Breeding. He has completed his from Rajasthan University of Veterinary and Animal Science, India. He has served in Rajasthan Dairy Sector for 13 years and has published more than 10 papers in reputed journals.
Email: lkgautam10@gmail.com
