Journal of Clinical & Experimental Pathology
Open Access

Abstract

Aims: This retrospective study aims to analyze tumors hot spot mutations frequency in KRAS, BRAF and Micro Satellite Instability (MSI) status of tumors in Algerian patients with advanced colorectal cancer, (CRC) which can predict prognosis and contribute to decisions on treatment strategies. Methods: KRAS exon 2, BRAF exon 15 were analyzed by direct sequencing of amplified PCR products in 102 tumors patients with advanced CRC cancer. MSI was determined using a panel of five mononucleotide markers (BAT25, BAT26, NR21, NR22 and NR24). Results: BRAF and KRAS mutations were detected in 4.9% and 31.3% of the tumors patients respectively. Activating mutations in codon 12 and 13 in KRAS was located in the right colon 40.6% vs. 25% in the left colon. (62.5%) with KRAS mutations are well or moderately differentiated. The amino acid changes are more frequently observed in codon 12 (29/32) than in codon 13 (3/32) and G12D (43.8%) is the most frequent mutation. BRAF v600E mutation is observed in proximal colon in 3 of 5 tumors (60%) in patients with older age >50 years (53.1%). BRAF wild type tumors (79%) were associated with MSI-H. Conclusion: The results of KRAS and BRAF mutation analysis could be used in the selection of Algerian patients with CRC for Anti- Epidermal Growth Factor Receptor (Anti-EGFR) therapy and MSI-H status associated with BRAF Wild type (W t) may be suggesting the possible presence of Hereditary Non Polyposis Colorectal Cancer (HNPCC) syndrome.

Biography

Email: kenzaberkane05@yahoo.fr