Journal of Biotechnology & Biomaterials
Open Access

Abstract

Withania coagulans is an immensely important medicinal plant. Over the years the plant has become critically endangered due to extremely poor germination along with low propagation rate in nature and over exploitation for various purposes. The present study reports an efficient method of mass propagation of W. coagulans through culture of cotyledonary segment and also comparative biochemical analysis of mother and tissue culture raised plants. Seed of W. coagulans were pretreated with 10% HCl solution for 10 minutes to enhance germination rate. Germinated seeds were surface sterilized and excised cotyledons were cultured onto MS+NAA (8 �¼M). Within 3-4 weeks of culture extensive callusing was obtained. Callus was further sub-cultured onto MS medium fortified with different PGR among which TDZ supplemented medium resulted in regeneration of shoot buds. After 8 weeks of culture a maximum of 35 shoot were regenerated onto MS+8 �¼M TDZ. Regenerated shoots were elongated onto basal MS medium and in vitro rooting was achieved onto �½ MS+20 �¼M IBA. About 68.8% plats survived during the process of acclimatization. GC-MS analysis of methanolic extract of leaves obtained from wild and in vitro regenerated plant of W. coagulans revealed presence of 45 and 56 phytochemical compounds respectively. 1-Penta decanamine, N, N-dimethyl; Palmitic acid, Methyl ester; Phthalic acid; butyltridecyl ester; 9-octadecenoic acid methyl ester (E); Betulin; Hexadecanoic acid, 2-hydroxy-1-(Hydroxy methyl) ethyl ester; Stigma sterol; Fuco sterol; Anthracane; Benzyl Benzoate and Tetradecanoic acid were major compounds present in mother plant whereas 9, 12 octadecadienoic acid (Z, Z); Hexadecanoic Acid, 2-hydroxy-1-(hydroxyl methyl) ethyl ester; n-hexadecanoic acid; Tetradecanoic acid; 1-Pentadecanamine, N, N-dimethyl; Benzoic acid, 4-ethoxy-ethyl ester; octanoic acid; Lycopene; gamma-tocopherol; Cholesterol and Stigmasta-5, 23-Dien-3-ol (3 Beta) were the major compounds present in tissue culture raised plants. Hence, the present study establishes tissue culture as an effective technique for mass propagation as well as enhanced production of bio-metabolites.

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