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Microbial bioremediation of industrial wastewater in common effluent treatment plant (CETP)

World Congress on Biotechnology

S S Sarnaik, P P Kanekar, G K Wagh, Snehal Bari, Nilesh Sonune and Amit Sinnarkar

MACS��? Agharkar Research Institute, India

Track 3: J Bioremed Biodegrad

DOI: 10.4172/2155-6199.1000001

Abstract
In the growing Indian economy both the Government and chemical industry is concerned about the treatment of the effluents. These concerns are exemplified due to the investment constraints on small scale and medium scale industries in erecting waste treatment plants. Under such a constraint CETPs are being considered as a common facility. Since the nature of effluents and their chemical composition is very diverse, CETPs face a grave challenge in treating such effluents. In the present study composite effluent from pesticide, pharmaceutical and dye industries received by a CETP was characterized. Some of the chemicals in the effluent include dyes namely CPC green G, pigment blue, red yellow HE4G, scarlet 4BS, acid fast red; pesticides namely triazolone, malathion, phorate, quinalphos, chlorpyriphos; pharmaceuticals namely pyrazinamide, dexamethazone etc. The CETP receives 1800 m3 of highly coloured effluent having pH 1.5. Chemical oxygen demand (COD) and ammoniacal nitrogen are the main concerns at the CETP. The bacterial consortium developed lowered the COD from an initial 8000 mg/l to 1500 mg/l at flask level and from 3000 mg/l to 1400 mg/l at a pilot plant of size 25 m3 on site. The consortium comprised of Ochrobactrum, seudomonas, Sphingomonas, Comamonas, Cupriavidus, Xanthomonas, Klebsiella, Bacillus, Flavobacterium, Aeromonas, Bacteroides, Ralstonia and Achromobacter. Acknowledgements: The authors thank authorities of Enviro Technologies Ltd., Ankleshwar, Gujarat for financial support, providing effluent samples and related information. Using the activated sludge process it was possible to play an important role in causing environmental pollution particularly water pollution due to large volume of effluent generated during the process and presence of toxic chemicals either in the form of unused raw material or unrecovered finished product. Effluent treatment plant thus becomes an unavoidable component of a chemical industry. Since, it is difficult to set up an effluent treatment plant independently for a small scale unit; existence of common effluent treatment plant (CETP) becomes useful for cluster of such small scale units. The wastewater in CETP is usually heterogeneous in nature. It is quite difficult to treat such type of mixed effluent using a single, pure microbial culture. MACS-Agharkar Research Institute (ARI), Pune is engaged in developing microbial cultures for treatment. The wastewater under study is from a CETP receiving waste from industries such as dyes and pigments, pesticides, pharmaceuticals, petrochemicals, etc. The wastewater is highly acidic in nature and dark in appearance with appreciablesettelable matter. It contains organic load in terms of Chemical Oxygen Demand (COD) to the extent of 8000 mg/L and ammoniacal nitrogen up to 900 mg/L. Bacterial consortium was developed using the bacteria isolated from the soil in the premises of CETP, and bacterial consortium used at site. The bacteria were belonging to genera Ochrobactrum, Pseudomonas, Sphingomonas, Comamonas, Cupriavidus, Xanthomonas, Klebsiella, Bacillus, Flavobacterium, Aeromonas, Bactroides, Ralstonia and Achromobacter. Except Bactroides, Ralstonia and Achromobacter, all other genera were found to be efficient in reducing the organic load and thus indicated their potential for bioaugmentation purpose.
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