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Overexploitation and hazardous consequences of use of fossil fuel led us to think about those solutions of energy which
should not only be renewable but carbon neutral also. A strong debate on the issue of future energy has provided some
solutions and bio-energy is one of the possible solutions as it complies the aim of renewability as well as there is no hazardous
consequences after its use. So development of such type of technologies that can provide bio-energy in future is need of the
hour. Microalgae have been considered as best source to provide bio-energy due to their enormous potential of growth, higher
content of sugars, lipids, pigments, easy to culture etc. But these organisms are well affected by different environmental factors.
So, it is necessary to optimize the culture conditions of microalgae so maximum biomass can be achieved. In this study, effect
of various components of light such as light color, intensity, period has been studied and their effects on biomass of micro alga
Chlorella minutissima were noticed. In addition of light, various carbon sources were also used to see their effect on biomass
yield. The type and shaking of culture vessel are also important factors that affect the biomass yield as naturally algae live in
running water. Thus experiments were also set up to see the effect of different culture vessels and shaking period. The light
conditions, carbon source and type of culture vessels and their shaking period have been optimized for this micro alga.