Journal of Biotechnology & Biomaterials
Open Access

Abstract

Acacia auriculiformis A. Cunn. ex Benth is an important fast growing exotic tree species in plantation forestry and also useful in pulp and paper industry. The genetic structure of forest trees and the pattern of genetic variation that are maintained in the successive generations are essentially a function of the mating systems. In the Institute of Forest Genetics and Tree Breeding, Coimbatore has been established seedling seed orchards of first and second generation trials. We have attempted a study on mating system of Acacia in two successive generations based on microsatellite markers. Three hundred half-sib progenies in the first and second generation orchards were studied for five different microsatellite loci and were profiled for detecting homozygous/heterozygous alleles. Specific amplicon sizes (150-250 bp) were sequenced, confirmed microsatellites and were submitted to NCBI (Accession No-KG699501.1; KG699563.1; KG699564.1). The microsatellite repeats as di-repeats viz., (CA)7, (TA)8, (CT)7 in three loci, and as tri-repeats such as (CTT)6, (TTC)7 in two loci. The allelic data were analyzed through maximum likelihood estimation of mating systems (MLTR) software. Single and multilocus out-crossing rate was estimated from the analysis. The range of the maternal, paternal genotypes among the individuals in the family was also studied in both generation orchards. The present study indicated high out-crossing rate (tm=0.98; SE=0.05; tm=1.0; SE=0.02) in first and second generation orchards population and it reflects that this species is highly out-crossed.

Biography

Email: Shanthia@icfre.org