Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
LC/MS/MS Quantitative Analysis Of Vitamin D: Hidden Analytical Errors Overlooked By Validation | 10239
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
Open Access
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Vitamin D deficiency is a widespread clinical problem and has been associated with many adverse health outcomes. Analysis
of Vitamin D2 (ergocalciferol) and D3 (cholecalciferol) and their major metabolites 25(OH)D2 and 25(OH)D3 has become
a high priority topic in clinical analysis. Since water loss is a favored process, in-source self dehydration of vitamin D can decrease
S/N and such an undesirable process was tightly controlled. In-source water loss was found to be insignificant (~8%) within
the assay linear range, using a methanol/water mobile phase under our experimental conditions. In contrast, if acetonitrile/
water was used, severe in-source water loss was observed and ions m/z 395.2 and m/z 383.2 resulting from analyte in-source
dehydration became predominant. When acetonitrile was used as mobile phase, and if only the transitions m/z 395.2�→?377.2 for
25(OH)D2 and m/z 383.2�→?377.2 for 25(OH)D3 were used for quantitation, the assay standard curve was still acceptably linear;
however the area ratio between analyte and its in-source water loss product was non-linear at different analyte concentrations.
The concentration-dependent water loss varied by 20%, resulting in a significant quantitation error in this provisionally valid
assay. For an acetonitrile-based mobile phase, both transitions (for analyte and for its in-source dehydrated product) should be
summed to maintain true full range assay linearity.
Biography
Eduard Rogatsky completed his M.Sc in physical chemistry from Belarus State University, Ph.D. in Bioanalytical chemistry from Bar-Ilan University
(Israel) in 1999, and postdoctoral studies at Albert Einstein College of Medicine, NY. He joined the faculty there in 2001, and is currently a Senior
Associate Scientist and Director of Mass Spectrometry in the Biomarker Analytical Resource Core Laboratory, Einstein-Montefiore Institute for
Clinical and Translational Research, Bronx, NY, USA
Relevant Topics
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals