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Kinetics of thermal inactivation of L-glutaminase from L-glutaminase from Bacillus cereus MTCC 1305
6th World Congress on Biotechnology
Priyanka Singh and Rathindra Mohan Banik
Banasthali University, India Banaras Hindu University, India
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Microbial glutaminase has many biotechnological applications in food and pharmaceutical industry. Enzyme deactivation is one of the major constraints in the development of biotechnological processes. This paper describes the study of thermal inactivation of purified L-glutaminase from Bacillus cereus MTCC 1305 at temperature ranging from 35-60�?�? C in term of deactivation energy (Ed), enthalpy (�?�?H), entropy (�?�?S) and free energy (�?�?G). The thermostability curve showed stability of this enzyme up to 50�?�? C with approximately 50% of activity remaining after incubation for 30 minute. The higher value of energy of deactivation (Ed=152.1038 KJ/K) and enthalpy (�?�?H��?149 KJ mol-1) indicates that more energy is required for thermal denaturation of this enzyme. The process of deactivation of L-glutaminase was found to be thermodynamically non spontaneous reaction with positive value of free energy. This enzyme showed more ordered state at all these temperatures with negative value of Entropy (�?�?S). Half-life (t1/2) of deactivation was found to be decreased with increase of temperature indicating higher degree of thermal inactivation process. These all thermodynamic parameters showed stability of this enzyme up to 50�?�? C with half-life deactivation (t1/2=34.478 minutes). The information of thermal characteristics of this enzyme could be a good candidate for industrial applications.Biography
Priyanka Singh has been currently working as an Assistant Professor in Department of Bioscience and Biotechnology, Banasthali University, India. She has been awarded Gold Medal for MTech degree (2008) from School of Biochemical Engineering, BHU. She has completed PhD from same department under supervision of Professor R. M. Banik in 2015. She has been awarded with renowned JRF (P)-Fellowship from DST-INSPIRE to pursue her PhD program. She has 9 research papers published in reputed journals to her credit. She has also presented papers in national and international conference and reviewed many papers as Reviewer of international reputed journals
Email: priyay20@gmail.com
