Journal of Biotechnology & Biomaterials
Open Access

Abstract

A total of 10 samples were collected from waste dump sites of Dhapa and Barrackpore Municipality and 9 isolates of bacteria were isolated using Nutrient Agar medium. The optimal cultural conditions, microbiological characteristics, biochemical characteristics, antagonistic and synergistic activities within the strains, tolerance to 5 heavy metals (Arsenic, Zinc, Lead, Mercury, Cadmium), their sensitivity towards four different antibiotics (Gentamycin, Oxytetracycline, Penicillin, Streptomycin) and production of extracellular enzyme of the bacterial strains were documented. Among the 9 isolates 6 exhibited protease production and 1 was selected for protease production with high titer value. All of the 6 strains capable of producing protease were also applied for waste degradation efficiency test. These results have increased the scope of finding industrially important bacteria from municipal waste dump sites and these isolates could be vital source for the discovery of industrially useful enzymes / molecules.

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