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In Association with
Increased lipid productivity of Scenedesmus dimorphus using optimized pulsed electric field
8th Euro Biotechnology Congress
Seong-Hyun Seo1,2, Hyun-Joon La1, Gang-Guk Choi3, Chuhyun Cho4, Ankita Srivastava1, Beom-Ho Jo5, Jae-Yon Lee1, Yun-Sik Jin4 and Hee-Mock Oh1
1Korea Research Institute of Bioscience and Biotechnology, Republic of Korea 2Hanyang University, Korea 3KAIST, Republic of Korea 4Korea Electrotechnology Research Institute, Republic of Korea 5National institute of Ecology, Republic of Korea
Posters-Accepted Abstracts: Biotechnol Biomater
Abstract
An alternative stress inducing method, a Low-Energy Pulsed Electric Field (LE-PEF) was used to improve the lipid productivity of microalgae cultures. A large shift in the Nile-Red stained peaks towards a higher intensity in fluorescentactivated cell sorting, suggestive of an increased neutral lipid content was observed when 10 kV LE-PEF pulses were applied to 800 mL batch cultures of Scenedesmus dimorphus. The optimal LE-PEF on-off cycle treatment for S. dimorphus was 10 s on and 60 s off for 15 min with 6 cycles per day. Under these optimal LE-PEF treatment conditions, S. dimorphus showed an overall 25.6% increase in lipid productivity. Therefore, based on these results LE-PEF can be regarded as a promising tool to avoid the lipid content-growth rate tradeoff and improve the lipid productivity of microalgae in various cultivation systems.Biography
Seong-Hyun Seo is a Master’s student of Hanyang University and Korea Research Institute of Bioscience and Biotechnology. He studies optimization of microalgae culture conditions for biodiesel production.
Email: heemock@kribb.re.kr
