Journal of Biotechnology & Biomaterials
Open Access

Abstract

Ramoplanin is a cell wall active lipoglycodepsipeptide antibiotic, which has bactericidal activity against a wide range of Gram-positive bacteria. It is considered as the last resort antibiotic for the treatment of VRE, MRSA and CDAD. It has been fast-tracked by FDA and is expected to be marketed in the forthcoming years. This urges low-cost, high efficiency ramoplanin production. Animal origin protein meals are used for animal feeding, enzyme production and bioactive material production. However, they are not widely used for biotechnological purposes without any chemical or enzymatic treatment. In this study, meat-bone meal, poultry-meal and fish meal were used instead of soybean meal for ramoplanin production by submerged fermentation of Actinoplanes sp. ATCC 33076. All animal origin protein meals stimulated specific productivity. The highest ramoplanin yields were determined as 406.805 mg/L in fish meal medium (3.89 fold increase), 374.218 mg/L in poultry meal medium (3.50 fold increase) and 252.130 mg/L in meat-bone meal medium (2.03 fold increase), respectively. However, the total yield of poultry meal medium was higher than fish meal medium, which is also low priced. Moreover, pH levels, extracellular protease, amylase and lipase activities, extracellular total protein and reducing sugar levels and intracellular amino acid levels were investigated. The limitation of carbon and nitrogen sources stimulated ramoplanin production. The intracellular levels of L-Phe, D-Orn and L-Leu were critical for ramoplanin production. In conclusion, fish meal and poultry meal were found effective for improvement of ramoplanin production. These protein meals can be applied for large-scale ramoplanin production.

Biography

Deniz Erkan has completed her Master’s degree in Biotechnology at Dokuz Eylul University, Izmir, Turkey in 2015 and she is currently a PhD student of Biotechnology at the same university.

Email: denizerkan87@hotmail.com