Journal of Biotechnology & Biomaterials
Open Access

Abstract

Pesticides such as organochlorides, organophosphates, carbamates are extensively used to increase crop production. On a flip side, the massive use of the pesticides has left the environment polluted with their concentration above permissible limits. Organophosphates are the most toxic among all the pesticides and affect nervous system by inactivating acetylcholinesterase (AChE) through irreversible binding. Various significant health threats posed by these chemicals foster the need for the development of portable, fast, selective, sensitive and cost effective methods for their sensing. The present work reports the electrochemical impedance spectroscopy (EIS) based ultrasensitive immunosensing of parathion using an antibody immobilized screen printed carbon electrode (SPCE). The experimental procedure involves the annealing of carboxylated graphene sheets on SPCE followed by the electrochemical deposition of amino-benzyl amine (ABA) resulting into the formation of an amine functionalized substrate which is subsequently used for achieving the oriented immobilization of parathion specific antibodies. The configured micro-device was used for qualitative and quantitative detection of parathion. FTIR and impedance measurements were performed to characterize the involved processes of functionalization, immobilization and sensing. The values of charge transfer resistance (RCT) were determined at each step. The data revealed the detection limit of the sensor=0.1 pg/mL. The proposed detection platform can be utilized for determining concentration of pesticides in water and food samples with high sensitivity and selectivity.

Biography

Email: jyotsanamehta1@gmail.com, dr.akashdeep@gmail.com