Journal of Infectious Diseases & Therapy
Open Access

Abstract

Influenza virus makes a large impact on public health. Annual influenza epidemics cause of death worldwide by almost 250 thousand is considered. Due to permanent mutations in the genome of the virus and the perpetual possibility of producing new viruses that occur as seasonal or pandemic flu, producing a vaccine for this virus is very important. According to the research and understanding of the genome of this virus and the use of genetic engineering techniques, universal vaccine produce is not out of reach. M2e is a conserved epitope that exists among the epitopes candidates for the vaccine against influenza. In addition to that this influenza virus region is antigenic, it is similar in the majority of flu strains and it is protected in some strains with minor differences in amino acid. It does not count appropriate stimulus to the immune system because this peptidic region is too short. For this reason, a molecular adjuvant called FliC was used. In this study, the piece consists of three sequence repeats of the M2e epitope attached to FliC, the molecular adjuvant, (3M2e.FliC) then transferred the recombinant plasmid to E. coli strains (BL21 and ER2566), we compare the protein expression in two strains. Immunoinformatics analyzes confirmed that in this recombinant protein, M2e and FliC epitopes are recognized by the immune system and they are existing at the protein surface and available for the immune system. From other activities performed in this study was Three-dimensional modeling of 3M2e.FliC recombinant protein that in this section, a new modeling method was introduced for recombinant protein modeling that provides better results than usual modeling methods.

Biography

Email: seyedmostafa.jalili@gmail.com