Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 3314

Journal of Biotechnology & Biomaterials received 3314 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • ICMJE
Recommended Journals
Share This Page

Identification of triticale- Rye hybrids with ISSR-PCR markers application

5th World Congress on Biotechnology

Tomasz Warzecha, Agnieszka Sutkowska and Halina Góral

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.027

Abstract
The F1 hybrids between maintainers of the cms- Triticum timopheevi system in triticale and maintainers of the cms-Pampa system in rye were obtained and their hybrid origin was verified. The F1 hybrids were selfing and maintaining ability effect of F2 individuals in triticale cms lines with T. timopheevi cytoplasm, were tested. After pollinating over seven thousands flowers of triticale with rye pollen, ten hybrid grains were obtained (0.14%). Their hybrid origin was verified by PCR using ISSR (Inter Simple Sequence Repeats) primers. Our sets primers produced 24 to 49 amplification fragments of different lengths. Among them, several fragments were monomorphic, i.e., common to maintainers of triticale, maintainers of rye and their F1 hybrids. In most primer-genotype combinations from one to four fragments were specific to the rye maintainers and the F1 hybrids. These data provide evidence for the hybrid character of the F1 plants. These fragments were not observed in female triticale maintainers. It is worth noting that in most of the studied hybrids more PCR fragments were obtained in female parents compare to male parents, this is most noticeable in the case of primer ISSR03. The most informative of the primers was ISSR 03 that generated seven polymorphic fragments that were present in male parents and the hybrids, and 18 fragments that were present in female parents and the hybrid. Fourteen F2 individuals (7.8%), as well as their S1 and progeny, generated stable male sterility in F1, BC1 and BC2 generations possessing T. timopheevi cytoplasm.
Biography
Tomasz Warzecha has completed his PhD in 2001 from Agricultural University in Krak ó w, Poland. He has participated at the International Postgraduate Course on Biotechnology in Agriculture, Plants and Microorganisms at the Hebrew University of Jerusalem. Additionally completed the Pedagogical Studium majored in Biology and Chemistry at Jagiellonian University in Krakow, Poland. He has worked in a project focused to examine natural variation in the recombination pathways in maize at the Department of Plant Breeding and Genetics at Cornell University, Ithaca, USA. At present he is Research Associate at the Department of Plant Breeding and Seed Science in Krakow.
Top