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Identification of QTLs for morpho-physiological traits in WL711/C306 wheat RIL population under water deficit stress environments
6th World Congress on Biotechnology
Kalpana Singh, Neeta Dwivedi and Renu-Khanna Chopra
Indian Agricultural Research Institute, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Drought is by far the most important environmental stress in agriculture and many efforts have been made to improve crop productivity under water-limiting conditions. A population of 206 recombinant inbred lines (RILs) derived from WL711/C306 was phenotyped for flag leaf area (FLA), plant habit (PHBT) and cell membrane stability (CMS) under WDS and irrigated environment. The RIL population showed considerable variation, normal distribution and transgressive segregation for FLA, PHBT and CMS under WDS. The genetic linkage map of WL711/C306 RIL population was constructed comprising of 346 markers. The total map distance was 4526.8cM with an averaged interval of 12.9cM between adjacent markers. Major consistent QTL for FLA, PHBT and CMS were identified on chromosomes 2DS, 3BS, 4BL and 7AL in the WL711/C306 RIL population under WDS. The Major QTL�s qFLAWD.2D.1 and qCMSWD.3B.1 were located on chromosome 2DS and 3BS respectively with positive allele being contributed by C306, a drought resistant parent accounting for a large proportion of phenotypic variance. Two candidate genes Ghd7 for grain yield and heading date and OsCDK4 for calcium dependent protein kinases were identified in the 2DS and 3BS QTL regions on comparison with gene content of rice chromosomes 7 and 1 respectively. Hence, after validation, markers associated with QTLs qFLAWD.2D.1 and qCMSWD.3B.1 may be used by wheat breeders in the marker assisted breeding for flag leaf area and maintain cell membrane integrity for contributing grain yield in wheat.Biography
Email: kalpanaschauhan@yahoo.co.in
