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Oil sludge components (polycyclic aromatic hydrocarbons, PAHs) have been found to be cytotoxic, mutagenic and potentially
carcinogenic and microorganisms such as bacteria and fungi can degrade the oil sludge to less toxic compounds such as
carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading potentials from the co-
composting of oil sludge and different animal manure. These bacteria were isolated on the mineral base medium and mineral salt
agar plates as a growth control. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost
treatments for identification using polymerase chain reaction (PCR) of the 16S rDNA gene with specific primers (16S-P1 PCR
and 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the gene
bank database. The phylogenetical analyses of the isolates showed that they belong to 3 different clades namely
Firmicutes,
Proteobacteria
and
Actinobacteria
. These bacteria identified were closely related to genera
Bacillus, Arthrobacter, Staphylococcus,
Brevibacterium, Variovorax, Paenibacillus, Ralstonia
and
Geobacillus species
. The results showed that
Bacillus species
were more
dominant in all treated compost piles. Based on their characteristics these bacterial isolates have high potential to utilise PAHs
of different molecular weights as carbon and energy sources. These identified bacteria are of special significance in their capacity
to emulsify the PAHs and their ability to utilize them. Thus, they could be potentially useful for bioremediation of oil sludge and
composting processes
Biography
Ubani Onyedikachi studied Biochemistry at Abia State University Uturu, Nigeria and Masters in Environmental Sciences at the University of South
Africa and currently doing Ph.D. in Environmental Science at the University of South Africa
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