Journal of Biotechnology & Biomaterials
Open Access

Abstract

Living tissues are composed of biologically and functionally different layers which forms in perfect hierarchy and harmony. To mimic these multilayer structures of the natural tissues, heterogonous tissue engineering term was introduced to the tissue engineering field very recently. Using this approach, different biochemical or topographical cues are localized in micro scale which may provide selective cell differentiation of a common stem cell and thus accelerate a new tissue formation similar to natural one. In our group, we designed many multi-layered structures for the repair of different tissues, including skin, tendon and abdominal wall. For mimicking natural skin tissue, we design two different bilayered constructs. To achieve the desired bilayered structure similar to skin, in both constructs freeze-drying and electrospinning methods were used consecutively. In another study, spiral shape aligned bilayered constructs were targeted to engineer tendon tissue. Nanofiber membranes from two different were obtained by electrospinning. To have the proper mechanical characteristics, these oriented membranes were combined in different forms as the fiber orientations are perpendicular or parallel to each other. The combined membrane layers were then rolled and glued at the edges in order to obtain spiral shape scaffolds. In our recent study, we aimed to develop multifunctional dual mesh for abdominal hernia repair. The developed mesh consists of a nanofibrous layer made of poly(glycerol sebacate)/poly(caprolactone) to support the healing of abdominal wall defect and a non-adhesive polyurethane layer to avoid the viscera. Our results from the studies revealed that, all designed constructs presented morphologic similarity to the targeted tissue.

Biography

Email: ktuzlakoglu@yalova.edu.tr