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Growth, acid production, bile tolerance and adherence to columnar epithelial cells of four species of bifidobacteria
14th Annual Congress on Gastroenterology & Hepatology
Ahmed Zahran
Minia University, Egypt
Posters & Accepted Abstracts: J Gastrointest Dig Syst
Abstract
Four species of bifidobacteria were studied for growth, acid production; bile acids tolerance and adherence to sheep epithelial cells were studied. The species examined were Bifidobacterium longum ATCC 15707, B. bifidum ATCC 2203, B. angulatum ATCC 2238 and B. breve ATCC 2258 were tested for growth and environmental conditions. The log phase of B.longum and B.angulatum were found to be after 14 hours phase whereas the log phase of the other two species were found to be after 17 hours of inoculation. Both B. longum and B. angulatum reduced the pH faster than both B. bifidum and B. breve. B. longum had the highest growth and B. breve had the lowest growth rate. All the studied species exhibited some degree of bile tolerance. B. longum and B. bifium were more resistant to bile acids than the other two species. Adhesion of the four species to the columnar epithelial cells of the small intestine of sheep was studied. All the tested species showed some degree of adhesion; however, B. longum adhered to the epithelial cells more than other three species.Biography
E-mail: asz52@yahoo.co.uk
