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Genetic engineering plays a unique and important
role in improving crop traits. We are developing an
engineering approach to improve biofuel production
as an alternative source of energy. Two of the most
important crops to be engineered in our laboratory are
switchgrass (Panicum virgatum) and sorghum (Sorghum
bicolor). To be successful in engineering these crops, it is
essential to develop an efficient Agrobacterium-mediated
transformation process in each of these crops. The
Agrobacterium-mediated T-DNA transfer offers several
advantages over other transformation systems. However,
in spite of previous reports, Agrobacterium-mediated
transformation of these two crops has been proven to
be difficult. Therefore, since our project started we have
optimized a number of critical conditions affecting
sorghum and particularly switchgrass transformation.
These conditions included the genotypes, cocultivation
temperatures and medium salt concentrations,
Agrobacterium strains, transformation vectors, selection
system and selective agents. These works have laid
good foundations for improved transformation of these
two crop species via Agrobacterium. Since improved
transformation systems were well established in our
laboratory, they have been used for research and
transformation services. For more information on how
to request switchgrass and sorghum transformation
services, please contact Dr. Zhanyuan Zhang.
Biography
Zhanyuan J. Zhang has completed his Ph.D. at the age of 40 years from University
of Nebraska-Lincoln, Nebraska, USA, and postdoctoral studies from the same
university. He is the director of Plant Transformation Core Facility at University of
Missouri, Columbia, Missouri, USA. He has published more than 22 papers in reputed
journals and has been selected as a permanent member of Madison Who?s Who, USA.
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