Journal of Biotechnology & Biomaterials
Open Access

Abstract

Integrons are strongly associated with the multidrug resistance seen in Gram-negative Bacilli in the hospital environment. These elements are able to capture and express gene cassettes encoding antibiotic resistance. The main aim of this study was to investigate the distribution of integrons in multidrug resistant diarrheagenic E. coli isolates to analyze the possible relationship between the antimicrobial resistance profiles and the integrons and to perform the docking of integron proteins. 80 diarrheagenic E. coli strains were isolated from children with diarrhea and examined for the presence of class 1, 2 and 3 integrons by real time PCR. 40 isolates from healthy children were also included as controls. Statistical analysis was used for the comparison of the categorical data. Class 1 integron was identified in most of the isolates while less than 50% isolates harbored class 2 integron and no class 3 integrons were detected in any of the isolate. Integrons were significantly associated with resistance to certain antibiotics including; Cefotaxime (P=0.01), Ceftazidime (P=0.006), Azetronam (P=0.046), Nalidixic acid (P=0.01), Gentamycin (P=0.001), Amikacin (P=0.01) and Piperacillin+tazobactam (P=0.037). Our study demonstrates the importance of integrons for the occurrence and transmission of multidrug resistance. Identical predominant class 1 and 2 integrons in E. coli servers� indicate horizontal transfer. This study emphasizes the alarming role of integrons in antibiotic resistance within diarrheagenic E. coli strains.

Biography

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