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In Association with
Genetic diversity in Pearl millet inbred lines using SSR markers
6th World Congress on Biotechnology
Sonali Sangwan, Shikha Yashveer and Ramesh Kumar
Chaudhary Charan Singh Haryana Agricultural University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Pearl millet [Pennisetum glaucam (L.) R. Br.] is a cereal cultivated in drought-prone semi-arid regions of Africa and the Indian subcontinent for food, forage and fodder. It is a principal source of energy, protein, vitamins and minerals for millions of poor people in the regions where it is cultivated. Pearl millet productivity can be increased by growing varieties/ hybrids with improved characters for which new allele combinations need to be discovered. All these generate the need for genetic diversity study. Morphological descriptors alone are not enough so, molecular markers have gained importance. PCR based markers like SSRs have been adjudged as more reliable for such studies. A set of thirty SSR markers were used to study diversity in thirty-six pearl millet genotypes. DNA from leaf tissue was isolated using CTAB method. PCR followed by PAGE revealed a good polymorphism among these genotypes. Mean allele per locus and PIC obtained was 10.5 and 0.8, respectively. UPGMA cluster analysis differentiated all the lines.Biography
Sonali Sangwan has completed her BSc Life Sciences in 2013 from Dayanand Postgraduate College, Hisar. She is currently pursuing her MSc working on genetic diversity study in pearl millet.
Email: sonalisangwan03@gmail.com
