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Extraction and characterization of chitosan nanoparticles from fish waste and its applications on waste water treatment of fish food processing industry
6th World Congress on Biotechnology
E Gokulalakshmi, M C Vanitha, K Ramalingam and N Srikumaran
Fish waste is considered to be one of the major bio-pollutants which are generally discarded in coastal regions through local
markets and fish processing industries. Nowadays, it is being eyed as a newer Bio-resource and few of the bio-products
commercially marketed are oil, bioactive peptides, collagen, chitosan and gelatin. In the present study we have used fish scales
waste generated through local market to synthesize chitosan nanoparticles. Antimicrobial activity for different pathogens like
staphylococcus aureus, Salmonella, Vibrio cholera, V. parahaemolyticus, E. coli, was performed out of these V. cholera, E. coli
showed a zone formation of 30 mm. Antioxidant assay various concentration of 20-100 was performed and the scavenging
activity of the sample is calculated as 11%. Haemolytic activity using goat�s blood is done in various concentration 101-108
activity showed is the haemolysis or the RBC break down is noted only from the 106 where as each cell carries the 2n amount
of haemolysis thus the factor is 26=64 Hu (haemolytic units). Further characterization of chitosan nanoparticles was performed
using SEM and FT-IR. Treating the obtained bio-products with effluent obtained from the fish processing industries is
studied. The pH and turbidity of the effluent was measured by checking the BOD and COD after treating the effluent with the
immobilized chitosan nanoparticles. The highest flocculation efficiency of chitosan observed under these conditions was 80%
COD removal and 90% of turbidity removal.