Journal of Biotechnology & Biomaterials
Open Access

Abstract

Metabolic syndrome (MetS) characterized by abdominal obesity, insulin resistance and dyslipidemia with increased risk for cardiovascular complications, fatty liver disease and cognitive dysfunction has reached epidemic proportions worldwide. Previous studies from our lab have shown an increase in Nor-epinephrine in plasma, brain and urine of high fat simple carbohydrate fed C57BL6J mice which served as a model for diet induced MetS. In the present study, we have tried to evaluate the status of adrenoceptors in the hypothalamus and pancreas of MetS induced C57BL/6J mice by real time PCR. The experimental group was divided into control and test and was fed with respective diet for a period of six months and samples were collected at regular intervals. Control was fed with standard lab chow and water ad libitum and test was fed with high fat simple carbohydrate diet and water ad libitum. The qReal time PCR was performed with hypothalamus and pancreas by using primers for �²3 and �±2A and SYBR green reagent according to the manufacturerâ��s protocol. A melt curve was also included to confirm the presence of non specific amplification and primer dimer formation. Fold change was found out by comparative Ct method. The study has shown an up-regulation of �²3 and �±2A adrenoceptor in pancreas after six months of feeding. In contrast to pancreas, there is down-regulation of �²3 and �±2A adrenoceptor in hypothalamus after six months of feeding. Understanding the adrenoceptor functional correlation in MetS may serve as a molecular target for drug discovery for metabolic syndrome.

Biography

Email: abraham.asha@gmail.com