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e study was purposed to develop a novel
cryopreserved agent (CPA) for platelets, to investigate
the morphology of cryopreserved platelets in diff erent
CPA and the CD62P expression on membrane of platelets
aft er stimulating by thrombin, as well as to compare
the eff ect of adding UDP�Gal on preserved effi ciency
of preservation solutions. A novel cryopreserved agent
consisting of 2% DMSO, thrombosol and UDP�Gal
was developed on basis of using higher concentration
of DMSO. Th e morphology of chilled platelets was
observed by transmission electron microscope and
compared with fresh platelets. Th e expression of CD62P
on the membrane of platelets was detected at 0, l, 3
months. Th e results indicated that the signifi cant eff ect
of cryopreservation on morphology of platelets was
found according to percentages of round, dendritic
and irregular shapes of cryopreserved platelets. Th e
protective eff ects of 2% DMSO+thrombosol and 2%
DMSO+thrombosol+UDP�Gal were better than
that of 5% DMSO. Compared with fresh platelets, the
expression of CD62P on platelet membrane decreased
obviously aft er cryopreservation, but not observed
diff erence at preservation for 1 month and 3 months, as
well as among 3 kinds of diff erent CPA. It is concluded
that the protective eff ects of 2% DMSO+thrombosol
and 2% DMSO+thrombosol+UDP�Gal on morphology
of platelets are similar, but better than that 5% DMSO.
Th e reaction of cryopreserved platelets to thrombin
decreases, while the signifi cant diff erence is not found
among these 3 kinds of CPA. Th e addition of UDP�Gal
to cryopreseved agents not show the protective eff ect on
platelets.
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