Journal of Biotechnology & Biomaterials
Open Access

Abstract

e study was purposed to develop a novel cryopreserved agent (CPA) for platelets, to investigate the morphology of cryopreserved platelets in diff erent CPA and the CD62P expression on membrane of platelets aft er stimulating by thrombin, as well as to compare the eff ect of adding UDP�Gal on preserved effi ciency of preservation solutions. A novel cryopreserved agent consisting of 2% DMSO, thrombosol and UDP�Gal was developed on basis of using higher concentration of DMSO. Th e morphology of chilled platelets was observed by transmission electron microscope and compared with fresh platelets. Th e expression of CD62P on the membrane of platelets was detected at 0, l, 3 months. Th e results indicated that the signifi cant eff ect of cryopreservation on morphology of platelets was found according to percentages of round, dendritic and irregular shapes of cryopreserved platelets. Th e protective eff ects of 2% DMSO+thrombosol and 2% DMSO+thrombosol+UDP�Gal were better than that of 5% DMSO. Compared with fresh platelets, the expression of CD62P on platelet membrane decreased obviously aft er cryopreservation, but not observed diff erence at preservation for 1 month and 3 months, as well as among 3 kinds of diff erent CPA. It is concluded that the protective eff ects of 2% DMSO+thrombosol and 2% DMSO+thrombosol+UDP�Gal on morphology of platelets are similar, but better than that 5% DMSO. Th e reaction of cryopreserved platelets to thrombin decreases, while the signifi cant diff erence is not found among these 3 kinds of CPA. Th e addition of UDP�Gal to cryopreseved agents not show the protective eff ect on platelets.

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