Journal of Biotechnology & Biomaterials
Open Access

Abstract

Nowadays, cancer is one of the diseases with the highest number of deaths worldwide. Glioblastomas are one of the most aggressive tumors of the central nervous system in adults. The average survival rate of patients diagnosed with this disease is only 3% in five years. The available treatments are only palliative, justifying the need of studies aimed at screening new compounds more effective for clinical use. The present study aimed to evaluate the antitumor potential of plant extracts of Annona coreacea in two human glioma lines, GAMG and U251. Cell viability was quantified by the reduction of MTT ([3-(4,5-dimethylthiazol-2yl)-2,5diphenyltetrazolium bromide) to formazan, its metabolization being proportional to the number of viable cells. The absorbance was measured by the spectrophotometer at wavelength 570 nm after 2 hours and 30 minutes incubation with MTT (2.5 mg/mL). The IC50 of all compounds was elucidated by a 24 hours dilution curve with concentrations (1 ���µg/mL, 7 ���µg/mL, 15 ���µg/mL and 25 ���µg/ mL). AcL3 was the most promising partition for U251, with IC50 equal to 1.355���µg/mL, and for GAMG AcL1 partition shown the best result, IC50 equal to 3.355 ���µg/mL. Migration assay was performed using the "wound-healing" methodology and aimed to evaluate the potential inhibition on cell migration of the partitions against U251 cell lines. Cells were treated with the IC50, pre-determined by MTT assay. Relative migration was estimated by the formula: % wound-healing percentage: (%) = 100 (AB)/A, where A is the width of the wounds before treatment, and B is the width of the wounds after treatment. The images were obtained at times 0, 12, 24 and 72 hours after treatment for measurements. AcL3 and AcL4 shown to be the best compounds when analyzed the inhibitory potential of the extracts on cell migration.

Biography

Lorena R Sousa has formed in Biology in 2015 at University Federal of Pampa located in SG, RS. Currently, she is pursuing her MS in Biochemistry and Molecular Biology at University Federal of São Joao Del Rey located in Divinopolis, MG. She works with glioblastomas cell lines, an aggressive tumor of the nervous central system of adults. In past, she worked with some other diseases such as Alzheimer's and Parkinson, using Drosophila melanogaster as an alternative model organism.