Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

Recently, disease such as obesity or hyperglycemia spreads by the disorder of westernization of the meal and the lifestyle in Japan, and life-style related diseases such as metabolic syndrome or diabetes attract its attention. In particular we investigated citrus fruit peels for diabetes in this study. We investigate here the peelings of Citrus dekopon [(Citrus unshiu × C. sinensis) × C. reticulata] which is one of citrus fruits, and examined alpha-glucosidase inhibitory activity to be known as a pharmacologic therapy of diabetes. Crushed the peelings of Citrus dekopon were extracted with 70% ethanol. The 70% ethanol extract was successively treated with 1-hexane and 1-butanol, and their extracts examined by alpha-glucosidase inhibitory activity. Remarkable inhibitory activity was separated by gel filtration and silica gel column chromatography. Four compounds were isolated from the peelings of Citrus dekopon , and their structures were established on basis of MS and NMR spectroscopy. They were naringin (1), poncirin (2), hesperidin (3), and 2, 3- dihydroxy -3, 4?- dimethoxy flavanone 7- O - rutinoside (4). As a result, compounds 1 and 2 were high α-glucosidase inhibitory activity. In conclusion, it might be used in general as food additives because remarkable alpha-glucosidase inhibitory activity was found from the peelings of Citrus dekopon.

Biography

Hiromi Kawabata is a graduate student of Kinki University, Department of Applied Biological Chemistry. She studies isolation and bioanalytical technique under Associate Professor Sawabe.