Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 3330
Journal of Biotechnology & Biomaterials received 3330 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Evaluation of stable reference genes and expression analysis of ATP1A1 gene in various tissues of riverine buffaloes (Bubalus bubalis)
6th World Congress on Biotechnology
Ramneek Kaur1,2, Monika Sodhi2, Vijay Lakshmi Sharma1, Ankita Sharma2, Sandeep Mann2 and Manishi Mukesh2
1Panjab University, India 2National Bureau of Animal Genetic Resources, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Riverine buffaloes are major livestock species contributing greatly to the dairy sector of our country. Till date, no suitable reference genes are available to normalize the transcriptional data across tissues of riverine buffaloes. Also the expression profiling of alpha1 sodium-potassium adenosine triphosphatase (ATP1A1) gene, an important candidate for its key role in adaptation and heat tolerance is not known across various tissues of riverine buffaloes. In the present study, a panel of 10 reference genes (GAPDH, ACTB, UXT, RPS15, RPL4, RPS9, RPS23, HMBS, �²2M and EEF1A1) were evaluated using three different algorithms, geNorm, NormFinder and BestKeeper to identify most stable reference in tissue samples (mammary gland, kidney, spleen, liver, heart, intestine, ovary, lung, muscle, brain and fat). The M-value given by geNorm ranged from 0.9797 (RPS9 and UXT) to 1.7362 (RPS15). From the most stable to the least stable, genes were ranked as: UXT/ RPS9>RPL4>RPS23>EEF1A1>B-ACTIN>HMBS>GAPDH>B2M>RPS15. NormFinder analysis ranked the reference genes according to the stability value as: UXT>RPS23>RPL4>RPS9>EEF1A1>HMBS>�²-ACTIN>�²2M>GAPDH>RPS15. Based on the crossing point SD value and range of fold change expression, BestKeeper analysis classified the genes from most to least stable as: RPS9> RPS23/UXT>RPL4>GAPDH>EEF1A1>�²-ACTIN>HMBS>�²2M>RPS15. Our data identified RPS23, RPS9, RPL4 and UXT gene to be the most stable and appropriate reference genes that could be utilized for normalization of transcriptional data in various tissues of buffalo. Our data showed expression of ATP1A1 in all the tissues though expression level varied in the following order: Kidney>heart>brain>lung>ovary>liver>fat>mammary glands>intestine>spleen> muscle. Relative expression of mRNA ATP1A1 in kidney was 4 folds higher expression thanin muscles. Hence the present study provides panel of stably expressed reference genes that can be utilized for functional studies in riverine buffaloes. In future such study will pave way to study functional aspects of buffalo specific genes including ATP1A1 that will enrich the bubaline genome.Biography
Email: dhillonramneek21@gmail.com
