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Plant growth promoting rhizobacteria are one of the most beneficial bacterial strains found in rhizosphere which has been
seen to improve crop yield leading to its extensive use in the field of organic farming. This study was carried-out to evaluate
nine rhizobacterial strains associated with Spinacea oleracea for different plant growth parameters under pot conditions. The
tested rhizobacterial strains were isolated in a previous study from soil that had been cultivated with Spinacia oleracea for
at least three consecutive seasons. This study is further extension of our work which involved the in vitro analysis of the
rhizobacterial strains for different plant growth promoting activities. In this pot study, all the rhizobacterial strains showed
positive result in terms of improvement in plant growth parameters when compared to that of control specimen. Strains R13,
R40, R50 and R53 showed best plant growth parameters. The shoot length of plants were inoculated with isolate R13, R40, R50,
R53 was 32 cm, 26.5 cm, 34 cm, 38.6 cm respectively, compared to control i.e., 21 cm, whereas root length for the same isolates
were 8 cm, 15 cm, 12.5 cm, 8 cm respectively, compared to control i.e., 9.5 cm. Number of leaves per plant for these strains
was 8 units, 6 units, 8 units and 7 units respectively, compared to control i.e., 6 units and leaf area was 20 cm2, 18.15 cm2, 20.07
cm2 and 13 cm2 respectively, compared to control i.e., 19.68 cm2. These results suggest that isolate R13, R40, R50, R53 may be
tested further for more plant growth promoting traits eventually developing it as potential soil inoculants in order to enhance
the growth of Spinacia oleracea.