Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 3330
Journal of Biotechnology & Biomaterials received 3330 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Evaluation of pathogenic potential and survival of Rhizoctonia solani isolates in rhizosphere of different wheat genotypes using conventional and real time PCR techniques
6th World Congress on Biotechnology
Bhupendra Singh Kharayat1, Jagat Kumar1, Kirti Rawat1, Sapna Sharma1, B M Bashyal1, S C Dubey2 and Rashmi Aggarwal1
1ICAR-Indian Agricultural Research Institute, India 2ICAR-National Bureau of Plant Genomic Research, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Rhizoctonia solani Kuhn (Teleomorph: Thanatephorus cucumeris Donk) is a ubiquitous soil-borne plant pathogenic fungus which causes significant yield losses in many agriculturally important crops. R. solani isolates were collected from the diseased root of mung bean (RDLM-1, RMPM-5, RMHM-6, RJHM-20 & RJKM-5), cowpea (RPBC-1) and rice (Rice-17) plants from different locations in India. Mass multiplication of isolates of Rhizoctonia solani was done on sorghum grains. Soil was mixed with fully colonized seeds of sorghum grains with R. solani isolates at 10 gm per 4 inches pots (containing 1 kg sterilized sandy loam soil in each pot). After proper mixing of inoculum in soil, 20 seed each of seven wheat cultivars viz., HD-2967, Agra Local, Suhzae, Ning-8133, Chiraya-3, Milan and Sonalika was sown. The pots were maintained at 28o C and 95% relative humidity under glass house conditions. Soil sampling were done at 0, 10, 21 days after sowing and after harvesting for quantification of inoculum level in soil. The soil DNA was extracted by using ZR Soil Microbe DNA Kit�. Ning-8133 wheat cultivar found resistant against six R. solani isolates (RDLM-1, RPBC-1, RJHM-20, RJKM-5, Rice-17 & RMHM-6) except RMPM-5. RJLM-20 and RMHM-6 were not able to produce symptoms after 14 days of sowing. Isolate RMPM-5 recorded most virulent in all cultivars among all the R. solani isolates. R. solani inoculum in the rhizospheric soil of different wheat cultivars were quantified at different time interval (0, 10, 21 days after sowing and after harvesting) using serial dilution and specific primer through real time PCR. Maximum concentration of inoculum was observed after 10 days of sowing. Inoculums started decline after 10 days of sowing and it was almost equal to the initial level after the harvest of the crop. Results indicated that R. solani disease incidence in wheat varies with cultivar and isolates of the pathogen. Wheat crop is not promoting R. solani after seedling stage and not reducing the inoculums concentration also. Information generated will be helpful for the management of pathogen in rice-wheat, mung bean-wheat cropping system.Biography
Email: bhupendrakharayat@gmail.com
