Journal of Biotechnology & Biomaterials
Open Access

Abstract

Selection of the genotypes using plasmid assisted technology provides an efficient and useful tool for elaborating genetic relationships among the genotypes. In the present study, 48 pea (Pisum sativum var sativum L.) genotypes (populations) obtained from different sources were analyzed through 20 RAPD, DNA markers for assessment of intraspecific DNA variations. The results revealed that significant variations were present in minor bands. Major bands also showed significant diversity. Considerable variations were also recorded in the density of some common bands. Maximum and minimum genetic diversity i.e., 80% and 20% was found among 8 and 23 comparisons respectively from banding profile. These variations can be used further for enhancing variability, a prerequisite for crop breeding. Phylogenetic clustering (through dendrogram analysis) of the genotypes revealed that genetic diversity is independent of origin or source of the genotypes. Forty eight genotypes of pea are clustered in three main groups A, B and C comprising 23, 5 and 20 genotypes, respectively. Group A1 and C1 included the most distantly related genotypes and hence can be recommended for breeding to obtain genetically diverse segregating populations.

Biography

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