Journal of Ecosystem & Ecography
Open Access

Abstract

Endophytes: the microorganisms that colonize the internal tissues of all plant species, are important components with yet undescribed novel natural products. Novel endophytes are associated with secondary natural products that have a tremendous impact on its application in pharmaceuticals and agriculture. The present study was directed towards isolation and molecular characterization of endophytic actinomycetes from four medicinal plants such as Cajanus lineata Wt. & Arn., Leucas ciliata Benth., Rauwolfia sp. Hook.F., Gomphostemma heyneanum Wall. Ex. Benth. found in Western Ghats of Karnataka. Isolations were made from leaves, stem as well as inflorescence of these plants. 82% from Cajanus lineata, 64% Leucas ciliata, 58% Rauwolfia sp, 26% Gomphostemma heyneanum actinomycetes isolates were obtained. The isolates were identified belongs to the actinomycetes genera based on spore or aerial mycelial characters and confirmed by molecular characterization of partial 16S rRNA gene sequences. The Streptomycete, Rhodococcus, Arthobacter were the most predominant actinomycete genera identified based on partial 16S rRNA gene sequences. The identified actinomycetes were subjected to fermentation and fermented product was screened for the inhibitors of α- amylase, the key enzyme in starch hydrolysis. These inhibitors inhibit the action of α- amylase results in reduction in starch hydrolysis which shows beneficial effects on glycemic index. The isolate A023 isolated from Leucas ciliata showed positive result for α- amylase inhibitors assay. The present study envisages the inhibitors of enzyme from microbial sources

Biography

Akshatha J.V completed M.Sc and M.Phil. degree from University of Mysore, currently doing Ph.D in Department of Studies in Botany, University of Mysore, Mysore, Karnataka and also working as Project fellow in UCG- MHRD sponsored Institute of Excellence project under the supervision of Dr. M .S. Nalini, Assistant Professor, University of Mysore. I secured Second Rank in Master of Science for securing highest mark in the university. Research work undertaken during M. Phil awarded Best Poster Presentation Award at the National Seminar organized by Indian Society of Mycology and Plant Pathology, Udaipur.