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To investigate the effects of sulfur on the nutrition value of DDGS for beef cattle, in vitro cultivation was conducted for 72 hours
with the rumen fluid collected from steers, setting different sulfur levels (0.346%, 0.692% and 1.038%) and various sulfur sources
(Na2SO4, Na2SO3, Na2S2O3 and Na2S), monitoring the fermentation parameters (dry matter digestibility, gas production and its rate)
and model predicted indicators (organic matter digestibility, metabolizable energy, net energy, microbial protein, partitioning factor
and gas yield). The results showed that, high sulfur level (0.692% and 1.038%) only decreased (P<0.05) asymptotic gas production
(b), while different sulfur sources resulted in various parameters, more specifically, sulfur from Na2SO4 and Na2S produced more gas
(P<0.05) with faster rate (P<0.01) of gas production than those of Na2SO3 and Na2S2O3, while Na2SO3 had the highest b and inverse
for Na2SO4 (P<0.01), which tended (P=0.09) to produced lower total volatile fatty acids than the others; sulfur from Na2SO4 and Na2S
also had a lower (P<0.01) DMD in 24 hours, MCP, PF24 and a higher (P<0.01) OMD, ME, NEm, NEg, GY24 than those of Na2SO3
and Na2S2O3. These results suggest that DDGS with different sulfur content ranging from 0.346% to 1.038% have a similar feed value
and dietary sulfur source exerts a great effect on its nutrition value for beef cattle.