Journal of Biotechnology & Biomaterials
Open Access

Abstract

Curcuma longa (Zingiberaceae), beauty of â??Indian Ayurvedaâ?? commonly known as â??Indian Saffronâ?? and â??Haldiâ?? in the Indian system of Medicines, is an erect perennial herb and grows as an annual crop. In tribal medicine, it has great importance as an anti-inflammatory, analgesic, antioxidant, antidiabetic, anticancer, antimalarial, anthelmentic, carminative, antiarthritic and many more which is proved by scientific evidences. Present research work deals with the development of in-vitro callus culture of such medicinally important plant and to find out the effect of explant source, age of explant, nutrient medium, various phytohormones and elicitation treatment on expression of secondary metabolites from callus. C. longa is propagated vegetatively in departmentâ??s research field and leaf sheath, leaf lamina, leaf base, lateral and apical buds of rhizome were used as explant for the present study. Explants were aseptically cultured on Murashige and Scoog (MS) medium, Gamborg (B5) medium, Whites medium and Woody plant medium supplemented with different concentrations of auxins and cytokinins like 2, 4-Dichlorophenoxyacetic Acid (2,4- D), Indole Acetic Acid (IAA), Naphthalene Acetic Acid (NAA), 6-Benzyl Adenine (6-BA) and Kinetin, individually as well as in combinations. The cultures were maintained at 25±2°C temperature and 16hrs photoperiod. Induced calli were then sub-cultured and maintained on the same medium. Calli were also subjected to elicitation treatment. After sufficient sub-culturing when no further growth of callus has seen, it is subjected to extraction and phytochemical screening.

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