Journal of Biotechnology & Biomaterials
Open Access

Abstract

Centella asiatica, also known as gotu kola, contains several active constituents and the most important bioactive compounds are triterpenoid saponins including asiaticoside, centelloside, madecassoside and asiatic acid. In addition, Centella sp. contains other components including volatile oils, flavonoids, tannins, phytosterols, amino acids and sugars. Due to its medicinal importance, this plant is being overexploited and to conserve this plant, it is necessary to micropropagate this plant in laboratory with high biomass yield. In the present investigation, comparative study of different accession of Centella asiatica for shoot initiation was performed. Optimizing the media for shoot culture is one of the critical parameter. So there is a need to find such conditions that will enhance the growth and phytocompounds production. For shoot culture, explant of different accession of Centella asiatica were inoculated in different media i.e., MS, Gamborgâ��s B5 and Nitsch which were supplemented with 1.5 mg/l BAP and BAP in combination with 0.5 mg/l NAA. The cultures were incubated at 25�±2�° C with photoperiod of 16 hours. After four week of incubation period best media was optimized. In future effect of different carbon sources on the growth and production of phytocompouds will be observed.

Biography

Email: nabneetab@dtu.ac.in