Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 7718

Journal of Bioremediation & Biodegradation received 7718 citations as per Google Scholar report

Journal of Bioremediation & Biodegradation peer review process verified at publons
Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • JournalTOCs
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Ulrich's Periodicals Directory
  • Access to Global Online Research in Agriculture (AGORA)
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Publons
  • Geneva Foundation for Medical Education and Research
  • MIAR
  • ICMJE
Share This Page

Effect of chitosan derivatives grafted poly(ethylene glycol) on the interaction with human DNA

7th International Conference and Exhibition on Biopolymers and Bioplastics

Hadi S. Al-Lami, Afrodet A. Salih and Sara H. Mutasher

University of Basra, Basra-Iraq

Posters & Accepted Abstracts: J Bioremediat Biodegrad

DOI: 10.4172/2155-6199-C1-012

Abstract
Chitosan is one of the natural materials used for gene delivery; this is why the current study focused on getting chitosan from chitin extracted from shrimp shells by known and modified chemical methods to obtain chitosan with a high Degree of Deacetylation to increase its solubility. It was characterized by Fourier Transform Infrared and intrinsic viscosity measurement in order to determine the degree of deacetylation and average molecular weight of purified Chitosan. Some chitosan derivatives were prepared by reaction of chitosan with maleic anhydride, adipic anhydride and sebacic anhydride in DMF to prepare N-malonyl chitosan, N-adipoyl chitosan and N-sebacoyl chitosan respectively, and because of low % yields of the resulting products, the three anhydrides were grafted to chitosan in toluene which is nearly doubled the products % yields. The acidic water-soluble derivatives of N-malonyl, N-adipoyl, and N-sebacoyl Chitosan were extended by grafting process in toluene with different poly(ethylene glycol) molecular weights (2000, 40000, and 20000 g/mole). Different %yields were obtained depending on the type of the derivative and the poly (ethylene glycol) chain length to evaluate their effect on binding to the human genomic DNA. The effect of these chitosan derivatives grafted poly (ethylene glycol) samples with human DNA has been explored by using electronic absorption spectroscopy and gel electrophoresis techniques. The observed changes in the physicochemical features of the polymers derivatives on binding to DNA suggest that they may bind to DNA with electrostatic interaction mode.
Biography
Top