Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Google Scholar citation report
Citations : 3314
Journal of Biotechnology & Biomaterials received 3314 citations as per Google Scholar report
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Dynamics of natural killer cells cytotoxicity in microwell arrays with connecting channels
Annual Conference and Expo on Biomaterials
Xu Yuanhao, S F Zhou, S Gopalakrishnan, Y W Lam and S W Pang
City University of Hong Kong, Hong Kong
ScientificTracks Abstracts: J Biotechnol Biomater
Abstract
Natural killer (NK) cells serve an important role in immune system by recognizing and killing potentially malign cells without antigen sensitization and could be important in cancer therapy. We have designed and fabricated microwell arrays with microchannel connections in polydimethylsiloxane (PDMS) substrates to study the interaction dynamics of NK-92MI cells with MCF7 breast cancer cells using time-lapse imaging by fluorescence microscopy for 15 h. Although cell seeding density is the same, NK cell cytotoxicity was found to be stronger in larger microwells, which is manifested as higher target death ratio (D=NMCF7 Death/NMCF7 Total) and shorter triggering time of first target lysis. Mirochannel connection between adjacent microwell of the same size increased the overall target death ratio by >10%, while connection between microwells of different sizes led to significantly increased target death ratio and delayed first target lysis in smaller mirowells. Our findings reveal unique cell interaction dynamics such as initiation and stimulation of NK cell cytotoxicity in a confined microenvironment, which is different from population-based study. The results could lead to a better understanding of the dynamics of NK cell cytotoxicity.Biography
Xu Yuanhao has completed his BEng and MSc degree at City University of Hong Kong and he is currently pursuing his PhD study at the same university. He is currently a Member of Centre for Biosystems, Neuroscience and Nanotechnology.
Email: yuanhaoxu617@gmail.com
