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In Association with
Development of RAPD-sequenced characterized amplified region marker for the identification of Viola serpens Wall. Ex Ging
6th World Congress on Biotechnology
Shipra Rani Jha
Jamia Hamdard, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Viola serpens Wall. Ex Ging is a glabrous perennial herb belongs to the family Violaceae. It is commonly known as Banfshah and is an antipyretic, antiseptic, demulcent, diuretic, emetic, expectorant and purgative in action. It is also useful in the treatment of asthma, bleeding piles, cancer of throat and skin diseases. RAPD-based SCAR marker was developed to identify Viola serpens. A putative 1130 bp marker specific to Viola serpens was identified using RAPD technique. This RAPD-amplicon was then sequenced and cloned. A 1130 bp polymorphic band obtained with OPAA-8 primer which was specific to Viola serpens accessions. A pair of SCAR primers (VioS F & VioS R) based on the sequences of unique RAPD amplicon was designed. The SCAR primers produced a specific 280 bp amplicon in authentic V. serpens and not in the allied species. Amplification of genomic DNA of Viola serpens with the SCAR primers produce a clear, bright and distinct band in Viola serpens only. This marker can be useful in the authentication of genuine plant from the market samples.Biography
Email: shipraranijha@gmail.com
