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Development of molecularly imprinted polymer based solid phase extraction in combination with simultaneous derivatization and dispersive liquid-liquid microextraction of 3-phenoxybenzoic acid in urine followed by GC-MS analysis
Biodiversity & Sustainable Energy Development-2012
Abhishek Chauhan
Posters: J Ecosyst Ecogr
Abstract
An analytical method based on molecularly imprinted polymer (MIP) in combination with simultaneous derivatization and dispersive liquid-liquid microextraction (DLLME) of 3-phenoxy benzoic acid (3-PBA) in a single step has been developed followed by its analysis using gas chromatography-mass spectrometry (GC-MS). 3-PBA is a metabolite of cypermethrin normally found in urine, and it is said to be a biomarker for cypermethrin exposure. The method involves the selective extraction of 3-PBA using MIP-solid phase extraction, further preconcentration by using DLLME and simultaneous derivatization by ethyl chloroformate in order to carry out its analysis in GC-MS. Factors affecting the synthesis of MIP for 3-PBA were evaluated and the best monomer and cross linker were selected for the synthesis of MIP. Various parameters of MIP-solid phase extraction, derivatization and DLLME were optimized in order to achieve selective preconcentration of 3-PBA in urine. The developed method offers a good linearity with a R2 value of 0.9971 in the calibration range of 0.08-1.28 �¼g/ml in urine. Under optimized conditions, the recovery of 3-PBA in the urine sample was found to be in the range of 83-91%. Limit of detection and limit of quantification was found to be 0.025 �¼g/ml and 0.076 �¼g/ml. Intra and inter-day precision for five replicates in a day, and for five successive days was found to be <8%. The validated method successfully applied for the quantitative evaluation of 3-PBA in the urine of cypermethrin treated rats. The developed method was found to be simple, rapid, efficient and inexpensive and found wide application for the routine analysis of 3-PBA in urine or other biological samples by any clinical and toxicological laboratories involved in this type of studiesBiography
Abhishek Chauhan has completed his Masters in Forensic Science from Dr. H. S. Gaur University, Sagar (M.P) in 2009. Currently, he is pursuing his Ph.D in Analytical Chemistry from CSIR-IITR, Lucknow. His area of work includes development of molecularly imprinted polymer for the analysis of selected toxicants. He has published one paper in internationally reputed journal, and other one are under communication. He is expected to have another two papers by the end of 2012
