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In Association with
Development of glyphosate-tolerant transgenic potato plants harboring the G2aroA gene
Annual Biotechnology Congress
Arfan Ali
Four Brothers Grouplahore, Pakistan
Posters & Accepted Abstracts: J Biotechnol Biomater
Abstract
Glyphosate weed control is a very effective strategy to minimize cost and improve economic outcomes of world and Pakistan agriculture production. Development of glyphosate -resistant potato holds great promise. A new G2-aoraA gene from Pseudomonas florescence which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was transformed by using an Agrobacterium-mediated transformation into potato cultivate AGB-Red. Transgenic potato plants were generated vis node tissue culture method using kanamycin selection. 10 regenerated potato plants were obtained and allowed to grow normally in pots under normal conditions. The polymerase chain reaction (PCR), southern blotting and western blotting analysis confirmed that the target gene was integrated and expressed effectively into potato chromosomes at the very potential level. The glyphosate tolerance assay showed that transgenic potato had a high resistance level to glyphosate. Furthermore, potato plants treated with 50.0 mili mole of glyphosate could grow slowly and can develop tubers. It was concluded that transgenic potato may be used for cotton breeding research of glyphosate -tolerant potato.Biography
Arfan Ali is from Centre for Excellence in Molecular Biology, University of the Punjab and Biotechnology Lab Four Brothers Group, Lahore, Pakistan.
E-mail: arfan.ali@cemb.edu.pk
