Journal of Biotechnology & Biomaterials
Open Access

Abstract

Plant protection against H. armigera has been progressively reoriented from a therapeutic approach to a rational use of pesticide chemicals in which consumer health and environmental preservation prevail over any other productive or economic considerations. Beauveria bassiana and Metarhizium anisopliae are the most widely used biocontrol agents and their application on plants is foliage spray. Unlike chemicals these biocontrol agents need support even after their application to get established in targeted niche. To ensure the sustained availability of biocontrol agent?s requires several steps addressed to its isolation in pure culture and screening by means of efficacy bioassays performed in vitro and in vivo trials under real conditions of application and standardization of abiotic factors. For the commercial delivery of B.bassiana and M.anisopliae, these were produced on Agricultural wastes like wheat bran flakes supported excellent growth of both B.bassiana and M.anisopliae. Population of B.bassiana and M.anisopliae in colonized wheat bran are as >10 8 cfu/gm of the fermented substrate. The developed fungi are formulated by means of biocompatible additives to increase survival and to improve the application and stability of the final product. This study led to test the e ffi cacy of developed final product under laboratory and field trials for control of H. armigera. Cost ?effective Technologies of colonization of biocontrol agents on agricultural wastes are becoming popular and to improve plant health and yield, irrespective of crop.

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