Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

A method was developed for the determination of the major storage lipids, wax ester and triglycerides, in the copepod Calanus finmarchicus . A variation of the Folch method was used to extract the lipid. The method was scaled down to enable the extract ion of either pooled (~l mg) or individual (~200 �¼g) copepods. The major lipid classes were identified using TLC and quantified using HPLC coupled with evaporative light scattering detection. Analysis of laboratory reference materials indicated that this method underestimated the minor triglyceride component, but gave a good estimate of the major wax ester component. The fatty acid and fatty alcohol composition of the C. finmarchicus were determined following trans- esterification of the lipid extract in methanol. Fatty acids and fatty alcohols were initially identified by comparison with authentic standard and by mass spectroscopy. Using GC with flame ionisation detection the normalised area percentage of the fatty alcohols and fatty acid m ethyl esters was deter mined simultaneously in one run for either pooled or individual copepod samples. These methods were applied to C. finmarchicus collected from the Irminger Sea, North Atlantic in 2001 and 2002.

Biography